Mixed with Italian and Cherokee heritage, Kimber James was born and raised in South Florida. She made her exciting adult performing debut in Devil's Film's Transsexual Babysitters 4, and so impressed the producers of the movie that they made her its cover girl.

Since her overwhelmingly successful debut, Kimber has gone on to star in several other hit adult movies and started her own popular website, Ts-KimberJames.com. In addition, James was the first transsexual to be signed to a major adult agency, as well as the first to be on the cover of AVN Europe.

In 2010, Kimber was named AVN's Transsexual Performer of the Year. Not long thereafter, she took an extended hiatus from the industry, during which she completed her sexual reassignment, becoming entirely female. In 2013, she announced her return and the launch of her very own studio, Kimber James Productions.